CONTRAST Beta Release 1.60 (01/25/94)
Protein AutoAssignment Tools
CONTRAST (CONnectivity TRacing ASsignment Tools) is a text-based, interactive
program for automating protein NMR assignments using lists of NMR peak
frequencies and intensities. CONTRAST's macro-based approach makes it possible
to use data from any set of multidimensional experiments. CONTRAST scores the
ambiguity in the assignments that it makes, and it gives a list of alternate
assignments for each resonance and for each residue that it assigns. It is fast
enough that it can be run several times to distinguish between variant
procedures and data sets. Given data from enough 3D heteronuclear backbone
experiments, CONTRAST usually assigns backbone resonances in under 30 seconds
for proteins up to 20kDa.
The CONTRAST program, an online
help manual, sample macros, and general program information are all
available from an NMRFAM FTP site. If you wish us to send you the FTP account
name and password or a uuencoded copy of the CONTRAST SGI executable, please
fill out and send us a copy of the CONTRAST license agreement which can be
downloaded using this utility.