Purpose: This introductory, hands-on workshop will cover the basic steps involved in solving a protein structure by NMR. Using triple-resonance NMR data collected at NMRFAM on labeled ubiquitin, the staff will guide participants through the following steps of protein structure determination
1) Description of data collection (raw data will be provided)
2) Data processing
3) Peak Picking
4) Backbone Assignment
5) Sidechain Assignment
6) NOE Assignments
7) Automated Assignments
8) Three dimensional structure determination and refinement
Format: The workshop format will be short lectures followed by hands-on sessions in which participants will have time to perform each step of protein structure determination using data provided by NMRFAM. The staff will be available during the sessions to answer questions and assist participants.
Due to the complexity of solving a protein structure by NMR, this workshop is intended to give particpants a broad over view of the process so they can begin their own projects. It is expected that particpants will require further assistance to solve a structure on their own. The particpants were invited to contact NMRFAM staff in the future with any questions or problems they may have.
Participant requirements: Participants in this course are not expected to have prior experience with the basic steps of NMR structure determination. The workshop will cover the entire process and each session built upon the previous sessions. Due to the time required to solve a protein structure, particpants will be given the data needed for each step of the process. For example, raw data will provided for the data processing session, processed data will be provided for the peak picking session, peak-picked data will be provided for the backbone assignment session, etc.
Each participant is expected to bring their own laptop to the workshop.
Registration Fee: To be determined
Travel and lodging: Particpants will need to make their own travel arrangements.
Blocks of rooms will be reserved at one or two locations to be determined. Please return to this site for updates.
NMRFAM Protein Structure Determination Workshop
June 3-8, 2007
This introductory, hands-on workshop covered the basic steps involved in solving a protein structure by NMR and gave the participants an opportunity to practice these steps in hands-on sessions led by the NMRFAM staff.
NMR Data Collection and Analysis for Biological Structure Determination Workshop
June 6-9, 2006
This workshop offered a unique opportunity for participants to experience a combination of hands-on computational sessions, presentations, and discussions focusing on recently developed tools as well as emerging methods in NMR data analysis.
Cell-Free Protein Production Workshop
July 31- August 4, 2006
The Center for Eukaryotic Structural Genomics (CESG) and the Nuclear Magnetic Resonance Facility at Madison
(NMRFAM) held their first Wheat Germ Cell-Free Protein Production Workshop on July 30 - August 4, 2006, at the Department of Biochemistry at the University of Wisconsin-Madison in Madison, Wisconsin, USA.
The NMRFAM Protein Structure Determination Workshop will be June 3-8, 2007
MMCD – Madison Metabolomics Consortium Database – a freely-available database containing information about small chemical compounds of biological interest.
New instructions for Remote Users are now available.
The new PINE server incorporates PISTACHIO (assignments), LACS (checking of carbon-13 chemical shift referencing and possible outliers), and PECAN (secondary structure determination) to yield results consistent with all three.
